Tìm kiếm theo cụm từ
Chi tiết
Tên RNAi-mediated resistance to SMV and BYMV in transgenic tobacco
Lĩnh vực Sinh học
Tác giả Lo Thi Mai Thu 1 , Vi Thi Xuan Thuy 1 , Le Hoang Duc 2 , Le Van Son 2 , Chu Hoang Ha 2 and Chu Hoang Mau 3*
Nhà xuất bản / Tạp chí Crop Breeding and Applied Biotechnology; 16: 213-218, 2016 Brazilian Society of Plant Breeding. Printed in Brazil http://dx.doi.org/10.1590/1984-70332016v16n3a32 (SCI-E) Tập 16 Số 3 Năm 2016
Số hiệu ISSN/ISBN 1518-7853
Tóm tắt nội dung

oybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly
generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of  viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the con -trol plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam