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Tên THE CHARACTERISTICS OF GmCHI GENE ISOLATED FROM SOYBEAN CULTIVARS WITH DIFFERENT ISOFLAVONE CONTENT
Lĩnh vực Sinh học
Tác giả Le Thi Hong Trang 1,2 , Tran Thị Thanh Van 3 , Ho Manh Tuong 4 , Pham Thanh Tung 4 , Le Van Son 3 , Chu Hoang Mau 1*
Nhà xuất bản / Tạp chí Journal of Biology, Viet Nam, 38(2): 236-242; DOI: 10.15625/0866-7160/v38n2.7959 Tập 38 Số 2 Năm 2016
Số hiệu ISSN/ISBN 0866-7160
Tóm tắt nội dung

In this study, isoflavone content of five soybean varieties disseminated grown in North Vietnam have
been surveyed, and the GmCHI gene encoding chalcone isomerase, a key enzyme in metabolizing synthetic
isoflavone in soybeans, was analyzed. Content of isoflavone (daidzein, genistein) in 3-day-old germinated
seeds  of soybean cultivar DT26 was the highest (64.27 mg/100g) and higher 2.18 times and 2.45 times
compared with DT84 and DT2008 respectively. GmCHI gene isolated from mRNA of the soybean cultivars
DT26, DT51, DT2008, DT84 is 657 nucleotides in length, encoding 218 amino acids. Similarity coefficient of
nucleotide sequence of GmCHI gene of four soybean DT26, DT51, DT2008, DT84 ranged from 96.8% to
98.9% and similar to those of NM_001248290 on GenBank from 98% to 99%. Genetic distances of DT26
compared with DT51 DT26, DT2008, DT84 and other varieties based on nucleotide sequences of GmCHI
gene was 19.3% and based on the amino acid sequence deduced was 4.4%. Coding region of GmCHI gene
has been used as a material for design transfomation vector for the improvement of isoflavone content in
soybean by gene transfer technique

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