Recently, proteomics has become one of the most promising and powerful tools for the proteome identification
in  plant  cells.  In  this  study,  a  proteomics  approach  was  used  to  analyze  and  characterize  soybean  leaf  proteins
from  a  soybean  rust  (Phakopsora  pachyrhizi Syd.)  resistant  soybean  cultivar  DT2000.  Initially,  nine-day-old
leaf  proteins  were  extracted  from  cultivar  DT2000  and  then  separated  by  two-dimensional  polyacrylamide  gel
electrophoresis (2DE). The collected protein spots were cut out, treated, trypsin-digested, and analyzed by using
two-dimensional  nano-liquid  chromatography  (LC)  coupled  online  with  tandem  mass  spectrometry.  Results
showed  that  119  protein  spots  were  found  in  2DE  gels.  Of  these,  35  leaf  proteins  were  characterized  from  the
cultivar  DT2000.  The  identified  proteins  were  classified  into  nine  functional  groups  by  Gene  Ontology  as
follows:  photosynthesis  (29%),  energy  (17%),  glycolysis  (11%),  storage  (9%),  metabolism  (8%),  transport  (8%),
transcription (8%), disease/defense  (8%) and unknown (6%). Especially, out of 35 characterized proteins from
the cultivar DT2000, three proteins related to the defense and disease resistance were discovered. The results of
our  study  indicated  that  the  2DE  combined  with  nanoLC  and  coupled  with  tandem  mass  spectrometry (ESI  Q
TRAP MSMS) analyze was a promising approach for the identification of soybean proteins

Tải file Proteomic characterization of soybean leaf proteins from cultivar DT2000 with rust-resistant ability in Vietnam tại đây