The avian influenza virus H5N1 causes disease in avian of many countries, and has already caused more
than $10 billion in losses to poultry farmers. This virus genome consists of eight segments of negative­stranded
RNA, which code for 11 proteins. In there, matrix proteins (M), hemagglutinin (HA) and neuraminidase (NA)
are  important  proteins,  which  are  targets  for  vaccine  production.  Vaccination  is  considered  as  the  most
important  measure  to  prevent  pandemics.  Although  different  types  of  influenza  vaccines  including
“inactivated” vaccines, vaccines with immunopotentiators, live attenuated vaccines and DNA  vaccines have
been developed, scientists around the world are developing better vaccines, which are less expensive, easier to
store and deliver, safer and more efficacious. Plant produced oral vaccines are particularly attractive because
they are economical, easily adaptable to large­scale production, present a minimal risk of contamination with
animal or human pathogens, and are stable at room temperature for a long time allowing them to be used in
remote  areas.  This  paper,  ha1op  gene  have  successful  constructed  in  plant  expression  vector.  Protein  HA1
fused a mucosal adjuvant protein LTB and C­myc tag is expressed in high level in Arabidopsis seeds. These
results are the base for immunological evaluation and protection of orally administered seed produced antigens.
Morever, these resuls will be applied for other antigen of virus.

Tải file Expression of H5N1 virus antigen HA in soybean seeds tại đây