Scar tissue of peanut cultivar  L18was processed by dry blower during 9 hours and in combination with
gamma ray irradiation with dose  of  2 krad and results showed that  198 peanut lines have  been generated. By
evaluation  of  drought tolerance of selected peanut lines at the fifth generation, 3 peanut lines RM48, RM47
and  R46  with highly  drought  tolerance  were  chosen,  of  which  RM48  line  derived  from  callus  dehydration
tolerance  and  radiation  tolerance  proved  to  be  the  best  drought  tolerance.  Cystatin  gene  sequence  of  the
RM48  peanut  line  had  19  different  nucleotide  positions  compared  with  that  of  the  L18  cultivar  (original
cultivar with low drought tolerance) and had 23 different nucleotide positions compared with that  of the L23
cultivar (high drought tolerance). Cystatin of the RM48 line had amino acid sequence which was different at 6
and 7 positions compared with that of the cultivars L23 and L18, respectively. Based on evaluation of drought
tolerance and analysis  ofcystatin gene sequence, the peanut line RM48 was selected. This line  derived from
dehydrated callus of L18 peanut cultivar by 9 hour dry blow and gamma ray irradiation at the  dose of  2 krad.
The line RM48 with highly drought tolerance could be introducedto participate in field trial test to generate a
new drought-resistant cultivar.
Keywords: Cystatin gene, drought resistance, irradiation resistance, peanut, scar tissue, water loss resistance.

Tải file Tạo dòng lạc bằng công nghệ tế bào thực vật tại đây