Chi tiết| Tên | Purification and properties of an endoglucanase from Aspergillus oryzae VTCC-F045 |
| Lĩnh vực | Sinh học |
| Tác giả | Huu Quan Nguyen, Dinh Thi Quyen |
| Nhà xuất bản / Tạp chí | Tập 4 Năm 2010 |
| Số hiệu ISSN/ISBN | ISSN 1991-8178 |
| Tóm tắt nội dung | |
An extracellular endoglucanase (EG) from Aspergillus oryzae VTCC-F045 was purified 2.6-fold to homogeneity throughout Sephadex G100 gel filtration chromatography. Purified EG had aspecific activity of 40.36 U/mg protein and a molecular mass of 36 kDa. Optimum temperature and pH were 55°C and 5.5, respectively. The enzyme was stable at up to 55°C and in pH range 5.0-6.0
with a residual activity of over 60% for 6 hours treatment. Metal ions, EDTA, organic solvents and detergents inhibited the enzyme
An extracellular endoglucanase (EG) from Aspergillus oryzae VTCC-F045 was purified 2.6-fold to homogeneity throughout Sephadex G100 gel filtration chromatography. Purified EG had aspecific activity of 40.36 U/mg protein and a molecular mass of 36 kDa. Optimum temperature andpH were 55°C and 5.5, respectively. The enzyme was stable at up to 55°C and in pH range 5.0-6.0with a residual activity of over 60% for 6 hours treatment. Metal ions, EDTA, organic solvents anddetergents inhibited the enzymeTải file Purification and properties of an endoglucanase from Aspergillus oryzae VTCC-F045 tại đây |
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