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Tên Cloning and Designing Vector Carrying GmEXP1 Gene Isolated from Local Soybean Cultivar Sonla, Vietnam
Lĩnh vực Sinh học
Tác giả Lo Thanh Son, Le Van Son, Nguyen Vu Thanh Thanh, and Chu Hoang Mau*
Nhà xuất bản / Tạp chí International Journal of Bioscience, Biochemistry and Bioinformatics, Vol. 4, No. 3, May 2014 Tập 4 Số 3 Năm 2014
Số hiệu ISSN/ISBN 2010-3638
Tóm tắt nội dung

Abstract—Soybeans  (Glycine  max  (L.)  Merrill)  are
short-duration industrial crops which have high economic and
nutritional values, and play an important role in improving soil
fertility  and  sustainable  use  of  cultivated  land  resources.
Soybeans have low level of drought tolerance, thus it is of great
necessity  to  study  on  approaches  to  improve  their  drought
tolerance, including using genes related to the root elongation.
The GmEXP1gene which expresses expansin, a key protein in
cell expansion, is one of those genes. In this study, we amplified,
cloned and determined the  GmEXP1gene sequence from local
soybean SL1 cultivar with best vigorous root systems. GmEXP1
gene is 790 bp in length, encoding 255 amino acids. Transgenic
vector  carrying  GmEXP1 gene  has  been  designed  successfully
(pCB301-GmEXP1) and  transformed  into  tobacco  plants  (N.
tabacumK326). These results form the basis for generation of
transgenic  soybean  cultivars  overexpressing  GmEXP1,  aiming
to improve the drought tolerance based on the root elongation
of soybean cultivars in Vietnam

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