Abstract—Soybeans  (Glycine  max  (L.)  Merrill)  are  easily
infected  by  soybean  mosaic  virus  (SMV)  and  bean  yellow
mosaic  virus  (BYMV),  which  may  reduce  their  yields  from
66%-80%.  At  present,  preventive  measures  are  still  the  main
methods to reduce losses caused by these two viruses. RNAi is
thus  considered  as  a  modern  and  promising  technique  to
generate plants resistant to viruses. In this study, we cloned and
characterized  the  Coat  protein  (CP)  coding  gene  of  SMV
lines SL1 and SL2, which is 720 bp in length and encodes 240
amino acids. The two conserved regions of CP gene from these
two viruses (SMV and BYMV) were integrated into a 573 bp
fragment,  called  CPi(SMV-BYMV).  Vector  pK7GW-CPi
(SMV-BYMV),  carrying  the  CPi  (SMV-BYMV)  fragment  in
sense  and  antisense  orientation  separated  by  an  intron,  was
successfully  designed  by  Gateway  technology  (Invitrogen).
Once being transformed into plant cells, this vector is expected
to  be  transcribed  into  a  hairpin  RNA  structure  that  could
induce RNAi mechanism to degrade the CP genes of SMV and
BYMV, thus limit their infection. Therefore, this vector could
be  a  potential  tool  to  generate  transgenic  soybean  plants  that
are  resistant  to  both  two  virus  species  SMV  and  BYMV  in
Vietnam

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