Soybean (Glycine max (L.) Merrill) is one of the most widely-known and grown food crops in the world
because of its economic and high nutritious value. In Vietnam more specifically, the local cultivars play an
important role in the lives of the people living in the regions with water stringency. In this research, we have
studied the response of local soybean cultivars in Tay Nguyen (DL, ST, CNg, AZP) and control one (NH9) to
drought and to the cloning of a chaperonin gene. Surprisingly, the response of local cultivars with drought condition
was different. DL cultivar had the level of drought tolerance higher than ST, CNg, AZT and NH9 with NH9 as the
lowest. Therefore, DL and NH9 were chosen for cloning the chaperonin gene. Using molecular cloning method,
we had two sequences of chaperonin gene in DL (DL- Chap1, DL- Chap2) and one in NH9. These sequences
consisted of 1602 nucleotide that encoded a propolypeptide with 533 amine acids. Gene DL- Chap2 is similar to
the chaperonin gene in Cuc Vang (98.74% of similar coefficients) but different from Bonminori of Japan in six
amine acid positions. Among them are the two sites: (Ser99
Thr, Ser 280
Gly) that confirmed the changing
law of temperature tolerance enzymes

Tải file The characteristics of chaperonin gene isolated local soybean cultivars (Glycine max L. Merrill) grown in Tay Nguyen region, Viet Nam tại đây