Lipid-transfer proteins (LTPs) are basic, 9-kDa proteins present in high amounts in higher plants . LTPs
can  enhance  the  in  vitro transfer  of  phospholipids  between  membranes  and  can  bind  acyl  chains.  LTPs
secretion and location in the cell wall were suggested that LTPs play roles in cutin formation, embryogenesis
and the response of plants to droughty conditions. In this study, the gene encoding LTP2 isolated frommRNA
of  mungbean  cultivar  Myduc-Hanoi  (HN2)  was  used  to  design  binary  vector  carrying  35S  -  LTP  -  cmyc  -
KDEL  -  NOS  (pCB301  -  LTP)  construct.  This  cassette  was  transformed  into  tobacco  via  Agrobacterium tumefaciens.  The  presence  of  LTP  gene  in  transgenic  tobacco  plants  was  confirmed  by  PCR  using  specific
primers. The result showed that there were 6/6 lines of transgenic tobacco plants had positive PCR product
which is 350 bp in size corresponding to this of LTP gene.These results lead to further studies of LTPgene
overexpression and analysis of drought tolerance intransgenic tobacco plants.
Keywords: mungbean, LTP, vector, transgenic, PCR, tobacco

Tải file THIẾT KẾ VECTOR NHỊ THỂ MANG GEN MÃ HÓA LIPID TRANSFER PROTEIN 2 (LTP2) PHÂN LẬP TỪ ðẬU XANH (VIGNA RADIATAL. WILCZEK) tại đây